Xylella fastidiosa gene expression analysis by DNA microarrays

Xylella fastidiosa genome sequencing has generated valuable data by identifying genes acting either on metabolic pathways or in associated pathogenicity and virulence. Based on available information on these genes, new strategies for studying their expression patterns, such as microarray technology, were employed. A total of 2,600 primer pairs were synthesized and then used to generate fragment...

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IDENTIFICATION OF TRAITS OF XYLELLA FASTIDIOSA CONFERRING VIRULENCE TO GRAPE AND INSECT TRANSMISSION BY ANALYSIS OF GLOBAL GENE EXPRESSION USING DNA MICROARRAYS Project Leader:

Xylella fastidiosa (Xf) regulates virulence factors important in both virulence to grape as well as colonization of sharpshooter vectors via its production of a fatty acid molecule (known as DSF) whose production is encoded by rpfF. The RpfF homologue of Xf strains that cause Pierce’s disease (PD), synthesizes a fatty acid cell-cell signal (DSF) that is apparently similar to that produced by Xa...

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DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

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DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

متن کامل

DNA microarrays and Gene expression analysis

Expression analysis for the quantitative gene expression of many genes can be performed using either oneor two-colour fluorescent schemes. One-colour analysis is primarily used for arrays prepared by photolithography. Affymetrix ( http://www.affymetrix.com ) patented this process under the trade name GeneChipTM (Fodor, S.P.A. et al., 1995 ).In this method, expression profiles for each sample ar...

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Xylella fastidiosa gene expression analysis by DNA microarrays

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